Priti Agarwal

• Planned, modified and executed research techniques, procedures and tests- Cell culture, Luciferase Assay for Protein-Protein Interactions, Transient and Stable Cell line generation using lentiral sytem, Proximity labeling using Turbo-ID method to identify protein interactors, Gene Cloning using Gibson Assembly method, Co-immunoprecipitation and Western Blotting
• Gathered, arranged and corrected research data to create representative graphs and charts highlighting results for presentations.
• Recorded experimental findings in laboratory notebook to analyze and interpret experimental findings.
• Worked with principal investigators to coordinate qualitative research.

Gained expertise in 3D Organoid culture from mouse small intestine.

• Kept work areas extremely clean and sanitized properly to avoid errors in research.
• Reduced project discrepancies by optimizing quality control standards.

Joined Florian Greten's lab as a guest student to learn 3D organoid cell culture from mouse small intestine.

• Demonstrated respect, friendliness and willingness to help wherever needed.

Carried out sample preparation and western blots for Mass Spectrometry based Proteomics studies for research groups in NCBS and Instem Cell culture

• Followed up with clients to verify optimal customer satisfaction following support engagement and problem resolution.
• Conducted diagnostics to investigate and resolve problems and provide technical assistance and support.

Carried out - In vitro kinase Assay for melanoma implicated BRAF, Western blotting, Co-Immunoprecipitation, De novo gene cloning, Gateway cloning, Plasmid preps, cDNA synthesis, Site-directed mutagenesis.

• Received Bristol Myers Squibb R&D Star Award for my work on Kinase Assays.
• Achieved project objectives by troubleshooting experiments and testing practical and creative solutions.
• Designed and executed studies to support usability of solutions, analyze data and provide actionable recommendations to project team.
• Streamlined research processes to meet tight deadlines for multiple projects.
• Conducted independent research to validate efficacy of solutions, assimilate results and prepare written technical reports.
• Initiated, planned and executed research projects under supervision of senior scientist.

Completed project and submitted research thesis titled "Characterization of chromosome II mutations that affect mitosis-meiosis transition in C. elegans" . Learned

C. elegans germ cell development, RNAi screening in worms and worm genetics.

• Completed rigorous readings as directed to support unit learning goals.
• Participated in lecture classes and asked compelling questions to further solidify learning.
• Studied topics using scientific methods to contribute to ongoing research.
• Mentored other students to support collaborative learning and further promote educational unit objectives.