Sandip Chorghade
Urbana,IL
Summary
Over 15 years of experience in mammalian cell biology, RNA biology, and recombinant protein expression. Proven track record in creating stable reporter cell lines, optimizing transfection protocols, and developing novel gene expression systems. Expertise in AML12,HepG2, HEK293 cell handling, viral vector-based gene delivery, and regulatory-compliant experimental documentation. Skilled at setting up new platforms from scratch, leveraging entrepreneurial and leadership experience in academic and biotech environments.
Overview
8
8
years of professional experience
Work History
Research Scientist / Senior Postdoctoral Fellow
University of Illinois at Urbana-Champaign
01.2021 - Current
- Developed a novel in vitro transcription system for synthetic mRNA generation in mammalian cells.
- Engineered and optimized RNA-FISH HCR for fixed cardiomyocytes—applicable in precise RNA localization.
- Created AAV8/Lentivirus-based systems for in vivo gene knockdown and transgene expression.
- Expertly performed transfection and stable line generation in various cell types including HeLa, HEK293, and MCF7.
- Contributed to publication on poly(A) tail-based post-transcriptional regulation in cardiac hypertrophy.
Co-Founder
Wonder Bug Enviro
01.2019 - 01.2021
- Established a start-up in biotech solutions, including diagnostics and microbial applications.
- Led the design and prototyping of a point-of-care COVID-19 diagnostic kit; selected among Top 60 innovations by the Indian Government.
Postdoctoral Research Associate
Duke-NUS Medical School
01.2017 - 01.2019
- Developed a Lentivirus-based dual reporter system and engineered a stable MCF7 reporter cell line, enabling scalable drug screening.
- Performed mRNA stability and gene expression studies in hypoxia-modulated macrophages.
- Conducted siRNA screens and transient/stable transfections to interrogate key pathways.
Education
Ph.D. - Microbiology and Cell Biology
Indian Institute of Science
India01.2011
M.Sc. - Microbiology
University of Pune
India04.2004
B.Sc. - Industrial Microbiology
University of Pune
Pune,India01.2002
Skills
- Mammalian Cell Line Development (CHO, HEK293)
- Stable Cell Line Engineering (Reporter & Expression Systems)
- Transfection (siRNA, plasmid, lentivirus, adenovirus)
- Cell Culture & Clone Screening
- Recombinant DNA Technology & CRISPR tools
- Viral Vector Systems (AAV8, Lentivirus)
- SOP & Report Writing (ISO, cGMP, 21 CFR Part 820)
- Immunofluorescence, IHC, Polysome Profiling
Awards
Top 60 Finalist, MHRD COVID Diagnostic Challenge, India, CSIR Senior Research Fellowship (India), 2008, DST-JRF Research Fellowship, 2004, GATE Top 0.1% Rank Nationwide, 2005
Publications
§ Chorghade S , Joe Seimetz, Bo Zhang, Chaitali Misra, Subhashish Natua, Qinyu Hao, Kananganattu V. Prasanth, Xander Wehrens, Jiwang Chen, and Auinash Kalsotra. “Dynamic expression poly (A) binding protein N1 coordinate cardiac growth during development and disease. (2024) Circulation.
§ Dhillon P, Tandra V, Chorghade S, Nima D. Namsa, Lipika Sahoo and C. Durga Rao. Cytoplasmic re-localization and colocalization with viroplasms of host cell proteins, and their role in rotavirus infection(2018) J. Virol. 1128/12.
§ Chorghade S, Semetz, J, Emmons R, Jing Y, Bresson S, DeLisio M, Parise G, Quick A, Xander H. Wehrens T, Conrad N, and Kalsotra A. “Poly (A) tail length regulates PABPC1 expression and tunes translation during heart development and cardiac hypertrophy” (2017) ELife; 6:e2413 .
§ Aguero T, Jin Z, Chorghade S, Kalsotra A, Yang J. Novel Mechanism Regulating T in Germline through eIF3 Complex and Dead-end1” (2017) Development, 10.1242 .
§ Bhate A, Parker D, Bebee T, , Arif W, Rashan E, Chorghade S, Chau A, Lee J, Anakk S, Carstens R , Xiao X & Kalsotra A. “ESRP2 controls an adult splicing program in hepatocytes to support postnatal liver maturation” (2015) Nat. Communications, 6 .
Languages
English
Full Professional
Hindi
Native or Bilingual
Marathi
Native or Bilingual
Timeline
Research Scientist / Senior Postdoctoral Fellow
University of Illinois at Urbana-Champaign
01.2021 - Current
Co-Founder
Wonder Bug Enviro
01.2019 - 01.2021
Postdoctoral Research Associate
Duke-NUS Medical School
01.2017 - 01.2019
Ph.D. - Microbiology and Cell Biology
Indian Institute of Science
M.Sc. - Microbiology
University of Pune
B.Sc. - Industrial Microbiology
University of Pune
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