Hala Muhtasb

• Executed genomic-functional assays to evaluate tumor sensitivity to cancer therapies.
• Prepared tissue samples including fixation, embedding, microtomy, H&E and IHC staining.
• Prepared and handled chemotherapy and biotherapy for in vitro testing.
• Conducted DNA extraction, PCR/qPCR, and DNA library preparation.
• Assisted in approximately 14 NGS runs (iSeq 100 platform), supporting setup, QC, and preliminary data review.
• Performed testing and quality control using approved laboratory procedures and instrumentation.
• Performed routine lab and department maintenance to keep area clean and orderly.
• Maintained documentation on tests performed in lab.
• Cleaned and sterilized tools and equipment to maximize purity of specimens.
• Prepared reagents, media and solutions to use for laboratory testing and analysis.

• Performed high-throughput qPCR COVID-19 diagnostics using 96- and 384-well plates.
• Managed biological samples to maintain processing efficiency.
• Prepared reagents and master mixes ensuring precision and reproducibility.
• Operated and monitored automated laboratory robotics.
• Recorded and analyzed data to produce reports of results.
• Analyzed results using analytical software and created reports.
• Compiled and submitted diagnostic reports to the Ministry of Health with zero errors.
• Tracked collected specimens by initialing, dating, and noting times of collection.
• Actively participated in lab safety inspections, identifying potential hazards and implementing corrective actions promptly.
• Facilitated timely completion of experiments by maintaining a well-organized workspace and efficient workflow.
• Prepared samples for analysis by performing complex sample preparation tasks.
• Troubleshot and resolved equipment and process issues.
• Prepared reagents and solutions following standard laboratory formulas and procedures.

• Cell culture techniques :

• prepare growth media,

• split adherent cells

• thaw cells,

• cell quantification (count ),

• freezing,

• basic aseptic conditions,

• cell culture equipment

• Cytotoxicity assay (MTT)

• molecular techniques DNA EXTRACTION,

RNA EXTRACTION , PCR

• Participated in workshops and presentations related to projects to gain knowledge.