Ephrem Kassa
Senior Research Scientist
Ramat Gan
Summary
Senior research scientist with 4+ years of industry experience in design, conduct and analysis of interdisciplinary projects. Proven expertise in generating and applying new protocols and technologies. Demonstrated success in training and supervising a team of scientists for various molecular biology tasks. Specialization in molecular target deconvolution and screen hit validation using high-throughput imaging, cutting-edge techniques such as HCR sm-FISH and other complementary assays.
Overview
3
3
Languages
Work History
Senior Scientist
Anima Biotech
1 2020 - Current
- Target deconvolution team member and supervisor of the RNA surveillance sub-group
- High throughput imaging assays – with Semi or full automation protocols
- Experience with the following technical skills: Tissue culture (Cell transfection, Viral infection, Stable cell line generation), Molecular biology (RNA/DNA/protein extraction, fractionation, PCR/qPCR, WB), Utilization of research tools (Confocal microscopy, flow cytometry, and multiple liquid handlers). I also have extensive experience in the analysis of data generated from these assays and tools.
- Transferrable skills: Project management skills - initiating, planning, executing, as well as coordinating the work of my collaborators, to achieve specific goals within the specified time.
Education
Ph.D. - Cell and Developmental Biology
The Hebrew University of Jerusalem
M.Sc. - Cell and Developmental Biology
The Hebrew University of Jerusalem
B.Sc. - Biology
Tel Aviv University
Skills
Molecular target deconvolution specialist
Accomplishments
- Introduced and developed the following cutting-edge assays; mRNA pulldown protocols (HyPR-MS and TRIP), mRNA/protein half-life estimation (RoadBlock qPCR and FUNCAT), smFISH assays (Stellaris, HCR, BranchFISH, FISH-IF & FLARIM) and Target deconvolution assays (smiFISH, Timing and inhibitor similarity assays)
- Trained and supervised multiple scientists on the aforementioned assays
- Initiated and managed company-wide knowledge exchange platform – Beer & Paper
- Coordinated with data/IT team to introduce and employ RNAseq projects: MACE-seq and Iso-Seq
- Generation of massive amounts of imaging and sequencing data intended for AI training
Honors And Awards
- Dr. Willem Been Award for outstanding graduate students, 2017
Publications
- Type III secreted effectors that target mitochondria . 2021 Sep;23(9):e13352. Cell Microbiol doi: 10.1111/cmi.13352. Epub 2021.
- Enteropathogenic E. coli Hijacks Host Endosomes to Promote Endocytic Turnover and Breakdown of Surface Polarity, PLoS Pathog, 06/26/2019, https://doi.org/10.1371/journal.ppat.1007851, 31242273
- Stinging Tight Junctions with WASPs, Cell Mol Gastroenterol Hepatol, 01/03/2018, https://doi.org/10.1016/j.jcmgh.2017.12.007, 29675455
Timeline
Senior Scientist
Anima Biotech
1 2020 - Current
Ph.D. - Cell and Developmental Biology
The Hebrew University of Jerusalem
M.Sc. - Cell and Developmental Biology
The Hebrew University of Jerusalem
B.Sc. - Biology
Tel Aviv University
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